2 mω tungsten microelectrode Search Results


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Thomas RECORDING optrode (optical fiber + tungsten microelectrode of 0.5–0.8 mω, with a core diameter 120 μm)
A-F Representative micrographs of triple immunostaining of Vglut2 (pseudo-color in red with Alexa Fluor 647), Vgat (green with Alexa Fluor 488), and orexin (pseudo-color in white Alexa Fluor 594) in lateral hypothalamus of control ( A-C ) and Firoc ( D-F ) mice revealed a significantly decreased number of Vglut2 puncta together with increased Vgat puncta onto orexin neurons ( n = 100 orexin neurons/mouse, 3 mice per group; *** p < 0.001; Mann Whitney U Test). B , E 3D representation of an orexin neuron (from the inset in A and D ) using Imaris software. C , F , Surface area of the same orexin neurons shown in B and E showing Vglut (red points) and Vgat spots (green points). G Percentage of Vglut2 spots in orexin neurons from each experimental group ( n = 100 orexin neurons/mouse, 3 mice per group, *** p < 0.001; Mann Whitney U Test). H Percentage of Vgat spots ( n = 100 orexin neurons/mouse, 3 mice per group; *** p < 0.001; Mann Whitney U Test). I Excitatory/ inhibitory ratio ( E / I) is significantly decreased in Firoc mice ( n = 100 orexin neurons/mouse, 3 mice per group); *** p < 0.001; Mann Whitney U Test. Scale bars in A and D : 100 μm, and in B , C , E and F : 15 μm. J A cartoon showing the intracranial localization of the electrode of stimulation in LPO (inhibitory inputs) and the <t>optrode</t> (optogenetic blue LED + Tungsten recording electrode) used to identify orexinergic cells in the LH/PeF area. K Unitary activity of orexinergic neurons in Or-ChR and Firoc-ChR animals. BLUE light pulses elicited 0.9 ± 0.22 spikes/50 ms in orexinergic neurons of Control-ChR mice, and 1.1 ± 0.19 spikes/50 ms in orexin neurons of Firoc-ChR mice ( n = 11, and n = 14, respectively). Thus, orexin neurons of Control-ChR and Firoc-ChR do not display differences in basal conditions ( p = 0.7299). However, after LPO stimulation (inhibitory inputs) and optogenetic activation, control-ChR mice show a 13% inhibition (0.78 ± 0.19 spikes/50 ms; p = 0.1135), whereas Firoc-ChR display a 31% inhibition (0.76 ± 0.12 spikes/50 ms; ** p < 0.01; sex balanced, Two-way RM ANOVA, Sidak’s Multiple comparison tests). Scale bars in A and D : 100 μm, and in B , C , E and F : 15 μm.
Optrode (Optical Fiber + Tungsten Microelectrode Of 0.5–0.8 Mω, With A Core Diameter 120 μm), supplied by Thomas RECORDING, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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optrode (optical fiber + tungsten microelectrode of 0.5–0.8 mω, with a core diameter 120 μm) - by Bioz Stars, 2026-09
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Thomas RECORDING 2 to 10 microelectrodes, made of quartz-insulated, tungsten-platinum material
A-F Representative micrographs of triple immunostaining of Vglut2 (pseudo-color in red with Alexa Fluor 647), Vgat (green with Alexa Fluor 488), and orexin (pseudo-color in white Alexa Fluor 594) in lateral hypothalamus of control ( A-C ) and Firoc ( D-F ) mice revealed a significantly decreased number of Vglut2 puncta together with increased Vgat puncta onto orexin neurons ( n = 100 orexin neurons/mouse, 3 mice per group; *** p < 0.001; Mann Whitney U Test). B , E 3D representation of an orexin neuron (from the inset in A and D ) using Imaris software. C , F , Surface area of the same orexin neurons shown in B and E showing Vglut (red points) and Vgat spots (green points). G Percentage of Vglut2 spots in orexin neurons from each experimental group ( n = 100 orexin neurons/mouse, 3 mice per group, *** p < 0.001; Mann Whitney U Test). H Percentage of Vgat spots ( n = 100 orexin neurons/mouse, 3 mice per group; *** p < 0.001; Mann Whitney U Test). I Excitatory/ inhibitory ratio ( E / I) is significantly decreased in Firoc mice ( n = 100 orexin neurons/mouse, 3 mice per group); *** p < 0.001; Mann Whitney U Test. Scale bars in A and D : 100 μm, and in B , C , E and F : 15 μm. J A cartoon showing the intracranial localization of the electrode of stimulation in LPO (inhibitory inputs) and the <t>optrode</t> (optogenetic blue LED + Tungsten recording electrode) used to identify orexinergic cells in the LH/PeF area. K Unitary activity of orexinergic neurons in Or-ChR and Firoc-ChR animals. BLUE light pulses elicited 0.9 ± 0.22 spikes/50 ms in orexinergic neurons of Control-ChR mice, and 1.1 ± 0.19 spikes/50 ms in orexin neurons of Firoc-ChR mice ( n = 11, and n = 14, respectively). Thus, orexin neurons of Control-ChR and Firoc-ChR do not display differences in basal conditions ( p = 0.7299). However, after LPO stimulation (inhibitory inputs) and optogenetic activation, control-ChR mice show a 13% inhibition (0.78 ± 0.19 spikes/50 ms; p = 0.1135), whereas Firoc-ChR display a 31% inhibition (0.76 ± 0.12 spikes/50 ms; ** p < 0.01; sex balanced, Two-way RM ANOVA, Sidak’s Multiple comparison tests). Scale bars in A and D : 100 μm, and in B , C , E and F : 15 μm.
2 To 10 Microelectrodes, Made Of Quartz Insulated, Tungsten Platinum Material, supplied by Thomas RECORDING, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A-F Representative micrographs of triple immunostaining of Vglut2 (pseudo-color in red with Alexa Fluor 647), Vgat (green with Alexa Fluor 488), and orexin (pseudo-color in white Alexa Fluor 594) in lateral hypothalamus of control ( A-C ) and Firoc ( D-F ) mice revealed a significantly decreased number of Vglut2 puncta together with increased Vgat puncta onto orexin neurons ( n = 100 orexin neurons/mouse, 3 mice per group; *** p < 0.001; Mann Whitney U Test). B , E 3D representation of an orexin neuron (from the inset in A and D ) using Imaris software. C , F , Surface area of the same orexin neurons shown in B and E showing Vglut (red points) and Vgat spots (green points). G Percentage of Vglut2 spots in orexin neurons from each experimental group ( n = 100 orexin neurons/mouse, 3 mice per group, *** p < 0.001; Mann Whitney U Test). H Percentage of Vgat spots ( n = 100 orexin neurons/mouse, 3 mice per group; *** p < 0.001; Mann Whitney U Test). I Excitatory/ inhibitory ratio ( E / I) is significantly decreased in Firoc mice ( n = 100 orexin neurons/mouse, 3 mice per group); *** p < 0.001; Mann Whitney U Test. Scale bars in A and D : 100 μm, and in B , C , E and F : 15 μm. J A cartoon showing the intracranial localization of the electrode of stimulation in LPO (inhibitory inputs) and the <t>optrode</t> (optogenetic blue LED + Tungsten recording electrode) used to identify orexinergic cells in the LH/PeF area. K Unitary activity of orexinergic neurons in Or-ChR and Firoc-ChR animals. BLUE light pulses elicited 0.9 ± 0.22 spikes/50 ms in orexinergic neurons of Control-ChR mice, and 1.1 ± 0.19 spikes/50 ms in orexin neurons of Firoc-ChR mice ( n = 11, and n = 14, respectively). Thus, orexin neurons of Control-ChR and Firoc-ChR do not display differences in basal conditions ( p = 0.7299). However, after LPO stimulation (inhibitory inputs) and optogenetic activation, control-ChR mice show a 13% inhibition (0.78 ± 0.19 spikes/50 ms; p = 0.1135), whereas Firoc-ChR display a 31% inhibition (0.76 ± 0.12 spikes/50 ms; ** p < 0.01; sex balanced, Two-way RM ANOVA, Sidak’s Multiple comparison tests). Scale bars in A and D : 100 μm, and in B , C , E and F : 15 μm.
Diameter Lacquer Insulated Tungsten Microelectrode Mng Active/1 Mω, supplied by fhc inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A-F Representative micrographs of triple immunostaining of Vglut2 (pseudo-color in red with Alexa Fluor 647), Vgat (green with Alexa Fluor 488), and orexin (pseudo-color in white Alexa Fluor 594) in lateral hypothalamus of control ( A-C ) and Firoc ( D-F ) mice revealed a significantly decreased number of Vglut2 puncta together with increased Vgat puncta onto orexin neurons ( n = 100 orexin neurons/mouse, 3 mice per group; *** p < 0.001; Mann Whitney U Test). B , E 3D representation of an orexin neuron (from the inset in A and D ) using Imaris software. C , F , Surface area of the same orexin neurons shown in B and E showing Vglut (red points) and Vgat spots (green points). G Percentage of Vglut2 spots in orexin neurons from each experimental group ( n = 100 orexin neurons/mouse, 3 mice per group, *** p < 0.001; Mann Whitney U Test). H Percentage of Vgat spots ( n = 100 orexin neurons/mouse, 3 mice per group; *** p < 0.001; Mann Whitney U Test). I Excitatory/ inhibitory ratio ( E / I) is significantly decreased in Firoc mice ( n = 100 orexin neurons/mouse, 3 mice per group); *** p < 0.001; Mann Whitney U Test. Scale bars in A and D : 100 μm, and in B , C , E and F : 15 μm. J A cartoon showing the intracranial localization of the electrode of stimulation in LPO (inhibitory inputs) and the <t>optrode</t> (optogenetic blue LED + Tungsten recording electrode) used to identify orexinergic cells in the LH/PeF area. K Unitary activity of orexinergic neurons in Or-ChR and Firoc-ChR animals. BLUE light pulses elicited 0.9 ± 0.22 spikes/50 ms in orexinergic neurons of Control-ChR mice, and 1.1 ± 0.19 spikes/50 ms in orexin neurons of Firoc-ChR mice ( n = 11, and n = 14, respectively). Thus, orexin neurons of Control-ChR and Firoc-ChR do not display differences in basal conditions ( p = 0.7299). However, after LPO stimulation (inhibitory inputs) and optogenetic activation, control-ChR mice show a 13% inhibition (0.78 ± 0.19 spikes/50 ms; p = 0.1135), whereas Firoc-ChR display a 31% inhibition (0.76 ± 0.12 spikes/50 ms; ** p < 0.01; sex balanced, Two-way RM ANOVA, Sidak’s Multiple comparison tests). Scale bars in A and D : 100 μm, and in B , C , E and F : 15 μm.
Tungsten Microelectrodes Fhc 2–3 Mω At 1 Khz, supplied by fhc inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Marzhauser Wetzlar GmbH Co KG tungsten microelectrodes (1–2 m)
A-F Representative micrographs of triple immunostaining of Vglut2 (pseudo-color in red with Alexa Fluor 647), Vgat (green with Alexa Fluor 488), and orexin (pseudo-color in white Alexa Fluor 594) in lateral hypothalamus of control ( A-C ) and Firoc ( D-F ) mice revealed a significantly decreased number of Vglut2 puncta together with increased Vgat puncta onto orexin neurons ( n = 100 orexin neurons/mouse, 3 mice per group; *** p < 0.001; Mann Whitney U Test). B , E 3D representation of an orexin neuron (from the inset in A and D ) using Imaris software. C , F , Surface area of the same orexin neurons shown in B and E showing Vglut (red points) and Vgat spots (green points). G Percentage of Vglut2 spots in orexin neurons from each experimental group ( n = 100 orexin neurons/mouse, 3 mice per group, *** p < 0.001; Mann Whitney U Test). H Percentage of Vgat spots ( n = 100 orexin neurons/mouse, 3 mice per group; *** p < 0.001; Mann Whitney U Test). I Excitatory/ inhibitory ratio ( E / I) is significantly decreased in Firoc mice ( n = 100 orexin neurons/mouse, 3 mice per group); *** p < 0.001; Mann Whitney U Test. Scale bars in A and D : 100 μm, and in B , C , E and F : 15 μm. J A cartoon showing the intracranial localization of the electrode of stimulation in LPO (inhibitory inputs) and the <t>optrode</t> (optogenetic blue LED + Tungsten recording electrode) used to identify orexinergic cells in the LH/PeF area. K Unitary activity of orexinergic neurons in Or-ChR and Firoc-ChR animals. BLUE light pulses elicited 0.9 ± 0.22 spikes/50 ms in orexinergic neurons of Control-ChR mice, and 1.1 ± 0.19 spikes/50 ms in orexin neurons of Firoc-ChR mice ( n = 11, and n = 14, respectively). Thus, orexin neurons of Control-ChR and Firoc-ChR do not display differences in basal conditions ( p = 0.7299). However, after LPO stimulation (inhibitory inputs) and optogenetic activation, control-ChR mice show a 13% inhibition (0.78 ± 0.19 spikes/50 ms; p = 0.1135), whereas Firoc-ChR display a 31% inhibition (0.76 ± 0.12 spikes/50 ms; ** p < 0.01; sex balanced, Two-way RM ANOVA, Sidak’s Multiple comparison tests). Scale bars in A and D : 100 μm, and in B , C , E and F : 15 μm.
Tungsten Microelectrodes (1–2 M), supplied by Marzhauser Wetzlar GmbH Co KG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tungsten microelectrodes (1–2 m) - by Bioz Stars, 2026-09
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A-F Representative micrographs of triple immunostaining of Vglut2 (pseudo-color in red with Alexa Fluor 647), Vgat (green with Alexa Fluor 488), and orexin (pseudo-color in white Alexa Fluor 594) in lateral hypothalamus of control ( A-C ) and Firoc ( D-F ) mice revealed a significantly decreased number of Vglut2 puncta together with increased Vgat puncta onto orexin neurons ( n = 100 orexin neurons/mouse, 3 mice per group; *** p < 0.001; Mann Whitney U Test). B , E 3D representation of an orexin neuron (from the inset in A and D ) using Imaris software. C , F , Surface area of the same orexin neurons shown in B and E showing Vglut (red points) and Vgat spots (green points). G Percentage of Vglut2 spots in orexin neurons from each experimental group ( n = 100 orexin neurons/mouse, 3 mice per group, *** p < 0.001; Mann Whitney U Test). H Percentage of Vgat spots ( n = 100 orexin neurons/mouse, 3 mice per group; *** p < 0.001; Mann Whitney U Test). I Excitatory/ inhibitory ratio ( E / I) is significantly decreased in Firoc mice ( n = 100 orexin neurons/mouse, 3 mice per group); *** p < 0.001; Mann Whitney U Test. Scale bars in A and D : 100 μm, and in B , C , E and F : 15 μm. J A cartoon showing the intracranial localization of the electrode of stimulation in LPO (inhibitory inputs) and the optrode (optogenetic blue LED + Tungsten recording electrode) used to identify orexinergic cells in the LH/PeF area. K Unitary activity of orexinergic neurons in Or-ChR and Firoc-ChR animals. BLUE light pulses elicited 0.9 ± 0.22 spikes/50 ms in orexinergic neurons of Control-ChR mice, and 1.1 ± 0.19 spikes/50 ms in orexin neurons of Firoc-ChR mice ( n = 11, and n = 14, respectively). Thus, orexin neurons of Control-ChR and Firoc-ChR do not display differences in basal conditions ( p = 0.7299). However, after LPO stimulation (inhibitory inputs) and optogenetic activation, control-ChR mice show a 13% inhibition (0.78 ± 0.19 spikes/50 ms; p = 0.1135), whereas Firoc-ChR display a 31% inhibition (0.76 ± 0.12 spikes/50 ms; ** p < 0.01; sex balanced, Two-way RM ANOVA, Sidak’s Multiple comparison tests). Scale bars in A and D : 100 μm, and in B , C , E and F : 15 μm.

Journal: Molecular Psychiatry

Article Title: Insulin-like growth factor I mitigates post-traumatic stress by inhibiting AMP-kinase in orexin neurons

doi: 10.1038/s41380-022-01442-9

Figure Lengend Snippet: A-F Representative micrographs of triple immunostaining of Vglut2 (pseudo-color in red with Alexa Fluor 647), Vgat (green with Alexa Fluor 488), and orexin (pseudo-color in white Alexa Fluor 594) in lateral hypothalamus of control ( A-C ) and Firoc ( D-F ) mice revealed a significantly decreased number of Vglut2 puncta together with increased Vgat puncta onto orexin neurons ( n = 100 orexin neurons/mouse, 3 mice per group; *** p < 0.001; Mann Whitney U Test). B , E 3D representation of an orexin neuron (from the inset in A and D ) using Imaris software. C , F , Surface area of the same orexin neurons shown in B and E showing Vglut (red points) and Vgat spots (green points). G Percentage of Vglut2 spots in orexin neurons from each experimental group ( n = 100 orexin neurons/mouse, 3 mice per group, *** p < 0.001; Mann Whitney U Test). H Percentage of Vgat spots ( n = 100 orexin neurons/mouse, 3 mice per group; *** p < 0.001; Mann Whitney U Test). I Excitatory/ inhibitory ratio ( E / I) is significantly decreased in Firoc mice ( n = 100 orexin neurons/mouse, 3 mice per group); *** p < 0.001; Mann Whitney U Test. Scale bars in A and D : 100 μm, and in B , C , E and F : 15 μm. J A cartoon showing the intracranial localization of the electrode of stimulation in LPO (inhibitory inputs) and the optrode (optogenetic blue LED + Tungsten recording electrode) used to identify orexinergic cells in the LH/PeF area. K Unitary activity of orexinergic neurons in Or-ChR and Firoc-ChR animals. BLUE light pulses elicited 0.9 ± 0.22 spikes/50 ms in orexinergic neurons of Control-ChR mice, and 1.1 ± 0.19 spikes/50 ms in orexin neurons of Firoc-ChR mice ( n = 11, and n = 14, respectively). Thus, orexin neurons of Control-ChR and Firoc-ChR do not display differences in basal conditions ( p = 0.7299). However, after LPO stimulation (inhibitory inputs) and optogenetic activation, control-ChR mice show a 13% inhibition (0.78 ± 0.19 spikes/50 ms; p = 0.1135), whereas Firoc-ChR display a 31% inhibition (0.76 ± 0.12 spikes/50 ms; ** p < 0.01; sex balanced, Two-way RM ANOVA, Sidak’s Multiple comparison tests). Scale bars in A and D : 100 μm, and in B , C , E and F : 15 μm.

Article Snippet: An optrode (Optical fiber + Tungsten microelectrode of 0.5–0.8 MΩ, with a core diameter 120 μm; Thomas Recording) was used to record the evoked potential in the LH/PeF area (coordinates from Bregma: A,−1.95; L, 1.0 and depth, 4.0–4.5 mm).

Techniques: Triple Immunostaining, Control, MANN-WHITNEY, Software, Activity Assay, Activation Assay, Inhibition, Comparison