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MicroProbes for Life Science
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Thomas RECORDING
optrode (optical fiber + tungsten microelectrode of 0.5–0.8 mω, with a core diameter 120 μm) ![]() Optrode (Optical Fiber + Tungsten Microelectrode Of 0.5–0.8 Mω, With A Core Diameter 120 μm), supplied by Thomas RECORDING, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/2+m%CF%89+tungsten+microelectrode/optrode++optical+fiber+++tungsten+microelectrode+of+0+5+0+8+m%CF%89++with+a+core+diameter+120+%CE%BCm+/pmc09126821-358-1-16 Average 90 stars, based on 1 article reviews
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Marzhauser Wetzlar GmbH Co KG
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Image Search Results
Journal: Molecular Psychiatry
Article Title: Insulin-like growth factor I mitigates post-traumatic stress by inhibiting AMP-kinase in orexin neurons
doi: 10.1038/s41380-022-01442-9
Figure Lengend Snippet: A-F Representative micrographs of triple immunostaining of Vglut2 (pseudo-color in red with Alexa Fluor 647), Vgat (green with Alexa Fluor 488), and orexin (pseudo-color in white Alexa Fluor 594) in lateral hypothalamus of control ( A-C ) and Firoc ( D-F ) mice revealed a significantly decreased number of Vglut2 puncta together with increased Vgat puncta onto orexin neurons ( n = 100 orexin neurons/mouse, 3 mice per group; *** p < 0.001; Mann Whitney U Test). B , E 3D representation of an orexin neuron (from the inset in A and D ) using Imaris software. C , F , Surface area of the same orexin neurons shown in B and E showing Vglut (red points) and Vgat spots (green points). G Percentage of Vglut2 spots in orexin neurons from each experimental group ( n = 100 orexin neurons/mouse, 3 mice per group, *** p < 0.001; Mann Whitney U Test). H Percentage of Vgat spots ( n = 100 orexin neurons/mouse, 3 mice per group; *** p < 0.001; Mann Whitney U Test). I Excitatory/ inhibitory ratio ( E / I) is significantly decreased in Firoc mice ( n = 100 orexin neurons/mouse, 3 mice per group); *** p < 0.001; Mann Whitney U Test. Scale bars in A and D : 100 μm, and in B , C , E and F : 15 μm. J A cartoon showing the intracranial localization of the electrode of stimulation in LPO (inhibitory inputs) and the optrode (optogenetic blue LED + Tungsten recording electrode) used to identify orexinergic cells in the LH/PeF area. K Unitary activity of orexinergic neurons in Or-ChR and Firoc-ChR animals. BLUE light pulses elicited 0.9 ± 0.22 spikes/50 ms in orexinergic neurons of Control-ChR mice, and 1.1 ± 0.19 spikes/50 ms in orexin neurons of Firoc-ChR mice ( n = 11, and n = 14, respectively). Thus, orexin neurons of Control-ChR and Firoc-ChR do not display differences in basal conditions ( p = 0.7299). However, after LPO stimulation (inhibitory inputs) and optogenetic activation, control-ChR mice show a 13% inhibition (0.78 ± 0.19 spikes/50 ms; p = 0.1135), whereas Firoc-ChR display a 31% inhibition (0.76 ± 0.12 spikes/50 ms; ** p < 0.01; sex balanced, Two-way RM ANOVA, Sidak’s Multiple comparison tests). Scale bars in A and D : 100 μm, and in B , C , E and F : 15 μm.
Article Snippet: An
Techniques: Triple Immunostaining, Control, MANN-WHITNEY, Software, Activity Assay, Activation Assay, Inhibition, Comparison